Polyclonal Antibody Generation
BioGenes is your expert for high-quality polyclonal antibody development services. Our dedicated team has developed more than 10,000 polyclonal antibodies for more than 30 years. We offer a variety of host species, and our immunization protocols ensure fast results, high yields, and a superior quality.
Development Strategy
Every polyclonal antibody development can be customized according to your specific needs and can cover any or all of the workflow steps — consultation, peptide or protein antigen production, antibody generation and purification – for a variety of applications, e.g. for the development of an ELISA.
Phase 1 - Expert consultation
- Selection of species and immunization protocol
Phase 2 - Preparation of the immunogen
- Antigen preparation (e.g. peptide synthesis or antigen conjugation)
Phase 3 - Immunization
- According to the selected species and protocol
Phase 4 - ELISA titer tests
- Of pre-immune sera and antisera
Phase 5 - QC & shipping
- Of pre-immune sera and antisera, optional affinity purified antibodies
Approximate project duration: 5 weeks (high speed protocol for proteins).
Extended protocols available.
If you need more information, a specific offer or want to talk to an expert:
Animals and Protocols
All animals used for polyclonal antibody generation are housed in certified facilities in Germany demonstrating our commitment and responsibility for animal welfare.
Rabbits
We offer different immunization protocols for antibody generation in rabbits, depending on whether antibodies are needed in a short time, highly specific or large amounts. Depending on the antigen (protein or peptide) and the requirements, our protocol lengths differ. The times and yields are shown in the following tables.
Protein Immunization Protocols
For the immunization of rabbits with proteins, we offer different strategies depending on the objective pursued. Certain protocols can be adapted in consultation with our scientists. The quality of the serum is always controlled by ELISA. After delivery of the antiserum the animals can be further kept if choosing the protein protocols High Speed, High Quality or High Yield.
| Protocol | Antigen provided by the customer | Products sent to the customer | Expected time frame |
| High Speed Protein | 1 mg (≈ 1 mg/mL) | 1.5 mL pre-immune serum and approx. 15 mL antiserum per animal | 5 weeks |
| High Quality Protein | 1.5 mg soluble protein (purity at least 90%, conc. and at least 1 mg/mL) | 1.5 mL pre-immune serum and approx. 30 mL antiserum per animal | 8 – 9 weeks |
| High Yield Protein | 2 mg protein (≈1 mg/mL) | 1.5 mL pre-immune serum and approx. 60 mL antiserum per animal | 17 weeks |
| Complete & Easy Protein | 1.5 mg protein (≈1 mg/mL) | 1.5 mL pre-immune serum and approx. 90 mL antiserum per animal | 15 weeks |
Peptide Immunization Protocols
Our scientists will assist you in the selection of the sequence and conjugation to a carrier protein. We recommend a sequence of 15 amino acids, which will be synthesized and purified by our collaborators. Immunization is carried out according to the following protocols and the customer's requirements. Each protocol also includes the delivery of 1.5 mL of pre-immune serum.
| Protocol | Antigen provided by the customer | Products sent to the customer | Expected time frame |
| High Speed Peptide | 15 mg | 1.5 mL pre-immune serum and approx. 15 mL antiserum per animal, one peptide aliquot | 10-11 weeks |
| High Quality Peptide | 15 mg | 1.5 mL pre-immune serum and approx. 30 mL antiserum per animal, one peptide aliquot | 12-13 weeks |
| Peptide Mix | 15 mg | 1.5 mL pre-immune serum and approx. 15 mL antiserum per animal, one peptide aliquot | 10-11 weeks |
| Complete & Easy Peptide | 15 mg | 1.5 mL pre-immune serum and approx. 90 mL antiserum per animal, one peptide aliquot | 17 weeks |
| Phospho-Peptide | 15 mg | 1.5 mL pre-immune serum and affinity purified anti-phospho-peptide and anti-non-phospho-peptide antibodies, one peptide aliquot | 14 weeks |
Mice
Immunization of mice is a cost-effective way to generate polyclonal antibodies if only small amounts of antiserum are required. We conduct a quality control of the antiserum using ELISA, if antigen is suitable. Immunization of mice with peptide antigens is possible upon request.
| Protocol | Antigen provided by the customer | Products sent to the customer | Expected time frame |
| Standard protocol | 0.5 mg (≈ 1 mg/mL) | 0.2 - 0.4 mL antiserum per animal | 42 days |
Rats
Immunization of rats leads to higher amount of antiserum compared with mice and is still fast and cost effective. We conduct a quality control of the antiserum using ELISA, if antigen is suitable. Immunization of rats with peptide antigens is possible upon request.
| Protocol | Antigen provided by the customer | Products sent to the customer | Expected time frame |
| Standard protocol | 0.5 mg (≈ 1 mg/mL) | 2 - 4 mL antiserum per animal | 42 days |
Sheep & Goats
Immunization of goats or sheep lead to large amounts of antiserum and/or purified antibodies with manageable time frames and animal numbers. The immunization schedules can be modified according to your special requirements and the aim of your project. We conduct a quality control of the antiserum using ELISA, if the antigen is suitable. Animals will be kept after delivery of the antiserum.
| Protocol | Antigen provided by the customer | Products sent to the customer | Expected time frame |
| 42-Day Protocol | 11 mg protein (≈ 5 mg/mL) | Pre-immune serum and 100 mL antiserum per animal | 42 days |
| 105-Day Protocol | 21 mg protein (≈ 5 mg/mL) | Pre-immune serum and 400 mL antiserum per animal | 105 days |
Additional Services & Information
Below you can find additional information regarding services, resources and antigen requirements.
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