Please note: Changeover from Triton™ X-100 to TWEEN®20
Please note: Changeover from Triton™ X-100 to TWEEN®20 Read more

Performance Study

With 360-HCP ELISAs you have the possibility to identify the most suitable generic HCP ELISA kit for your specific process. The Starter Set (for CHO and for E. coli) provides all the necessary components, so that you can quickly and easily choose the most appropriate kit type.

Order your starter set in our new online shop now!

Alternatively, ask for BioGenes' Performance Study, where our scientists evaluate all of BioGenes' proprietary four kit types for you. The Performance Study includes the following service parts:

Performance study – part 1

Characterization of HCP-containing samples using BioGenes 360-HCP ELISA kits (optionally, we can include HCP ELISA kits from other manufacturers). Ideally, samples would be based on three in-process controls (IPCs) from different purification steps provided by the customer.

  • Preparation of 1D Western blots and immunostaining with antibodies from 360-HCP ELISA kits

  • Determination of HCP content of the three different IPCs by ELISA

  • Determination of ELISA accuracy using a spike-and-recovery protocol and the most suitable selected 360-HCP ELISA kit (also using Mock sample, if available)

  • Detailed report

Performance study – part 2

This part is based on the results from Part 1 of the performance study. To confirm the suitability of the selected generic HCP ELISA kit from Part 1, BioGenes offers the HCP coverage determination by comprehensive 2D analysis of a single process sample:

  • Coverage determination using 2D Western blot

  • Optional: Coverage determination by Immunoaffinity Chromatography (IAC) followed by 2D DIGE analysis

  • Detailed report