Sophisticated Generic HCP ELISA Kits and BlueCap Buffers
Sophisticated Generic HCP ELISA Kits and BlueCap Buffers

CHO|360-HCP ELISA

The CHO|360-HCP ELISA is an enhanced generic host cell protein (HCP) assay. It was designed to cover a broader spectrum of Chinese hamster ovary (CHO) host cell proteins (HCPs) than other commercially available generic HCP assays.

CHO|360-HCP ELISA is based on a unique assay design employing differently prepared HCP antigens and antibodies from two species:

  • Antigen is prepared from total and fractionated HCP obtained from mock fermentation of CHO-K1 and CHO-S 
  • Specific antibodies are generated by immunizing goats and rabbits
  • Approach results in four different HCP ELISA kits (type A to D) that together build up the enhanced generic CHO|360-HCP assay
     
Type Species HCP
Type A Goat Total HCP
Type B Goat Fractionated HCP
Type C Rabbit Total HCP
Type D Rabbit Fractionated HCP

Sensitivity of CHO|360-HCP ELISA kits (A to D)

  • LOD between 0.5 – 1,0 ng/mL
  • LOQ between 2 – 3 ng/mL
  • Working range 2 – 100 ng/mL

HCP recovery strongly depends on type of ELISA kit used

“There is not the one generic HCP ELISA suitable for all samples and users are well advised to test different kits before making their choice.”

To exemplify, we tested CHO|360-HCP ELISA kits (A to D) and a commercially available generic CHO HCP assay on the basis of a great number of mock CHO HCP samples. Figure 2 shows that in most cases the best recovery was estimated with one of the four CHO|360-HCP ELISAs kits. In case of sample 4, a recovery of > 90% was estimated with ELISA kit type D while ELISA kits A, B and C as well as the commercial kit were less sensitive with the latter recognizing only 20% of the host cell proteins.

Product overview

Performance Study

2D Western Blot

Are the HCP antibodies fit for purpose?

BioGenes offers the 2D analysis to estimate HCP coverage.

 

Consistent Quality

High quality and long-term availability needed?

BioGenes offers supply agreements for long-term delivery of ELISA kits.