Immunization of Rats using Proteins
Required amount of antigen per animal: at least 0.5 mg at ~1 mg/mL
Immunization of rats using a 42-day protocol
Delivery of pre-immune serum, test serum and 2 – 4 mL antiserum per animal
Quality control of the antiserum using ELISA, if antigen is suitable
Immunization of Mice using Protein
Required amount of antigen per animal: at least 0.5 mg at ~1 mg/mL
Immunization of mice using a 42-day protocol
Delivery of pre-immune serum and 0.2 – 0.4 mL antiserum per animal
Quality control of the antiserum using ELISA, if antigen is suitable