Immunization Protocols of Rabbits using Peptides
| Protocol | Antigen synthesized by BioGenes | Products sent to the customer | Expected time frame |
|---|---|---|---|
| High Speed Peptide | Peptide (15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier | 1.5 mL pre-immune serum and 20 mL antiserum per rabbit, one aliquot of the peptide | 10 – 11 weeks after ordering |
| High Quality Peptide | Peptide (15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier | 1.5 mL pre-immune serum and 40 mL antiserum per rabbit, one aliquot of the peptide | 12 – 13 weeks after ordering |
| Complete & Easy Peptide | Peptide (15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier | 1.5 mL pre-immune serum and approx. 90 mL antiserum per rabbit, remaining peptide | 17 weeks after ordering |
| Peptide Mix | Two peptides (each 15 mg, purity >80%), assistance in sequence selection and conjugation to a carrier | 1.5 mL pre-immune serum and 20 mL antiserum per rabbit, one aliquot of each peptide | 10 – 11 weeks after ordering |
| Phospho-Peptide | Phosphorylated and corresponding non-phosphorylated peptide (15 and 10 mg, respectively, purity >80%), conjugation to a carrier | 1.5 mL pre-immune serum and affinity purified anti-phospho-peptide and anti-non-phospho-peptide antibodies, one aliquot per peptide | 14 weeks after ordering |
High Speed Peptide
- Peptide synthesis (15 aa, 15 mg, at least 80% purity)
- Conjugation of peptide to a protein carrier
- Immunization of two rabbits according to a schedule over 42 days
- Delivery of pre-immune serum and 20 mL antiserum per rabbit
- Quality control of the antisera using ELISA
- The animals will be kept after delivery of the antiserum
High Quality Peptide
- Peptide synthesis (15 aa, 15 mg, at least 80% purity)
- Conjugation of peptide to a protein carrier
- Test of pre-immune sera and selection of suitable animals
- Immunization of two rabbits according to a schedule over 56 days
- Quality control of the antisera using ELISA
- Delivery of pre-immune serum and 40 mL antiserum per rabbit
- The animals will be kept after delivery of the antiserum
Complete & Easy Peptide
- Peptide synthesis (15 aa, 15 mg, at least 80% purity, quality control by HPLC, MS)
- Conjugation of peptide to a protein carrier
- Immunization of two rabbits according to a schedule over 91 days
- Quality control of the antisera (first bleeding) using ELISA
- Delivery of pre-immune serum, 80-100 mL antiserum per rabbit
Please note: This protocol cannot be altered, and the rabbits are not further kept!
Peptide Mix
- Peptide synthesis of two peptides (15 aa, 15 mg, purity at least 80% each)
- Conjugation of peptides to a protein carrier
- Immunization of two rabbits according to a schedule over 42 days
- Delivery of pre-immune serum and 20 mL antiserum per rabbit
- Quality control of the antisera using ELISA (both peptides)
- The animals will be kept after delivery of the antiserum
- Synthesis of the phosphorylated peptide (12 aa + one phosphorylation, 15 – 20 mg, at least 80% purity, quality control by HPLC and MS)
- Synthesis of the corresponding non-phosphorylated peptide (12 aa, 10 mg, at least 80% purity, quality control by HPLC and MS)
- Conjugation of the phosphorylated peptide to a protein carrier
- Immunization of two rabbits according to a schedule over 42 days
- Taking of pre-immune serum and 20 mL antiserum per rabbit
- Quality control of the antisera using ELISA against the phosphorylated peptide
- Affinity purification of up to 2 x 20 mL antiserum using three affinity columns: one loaded with Protein A, one loaded with the phosphorylated peptide and loaded with the unphosphorylated peptide
- Delivery: samples of peptides, pre-immune serum, test serum, specific anti-phophopeptide and anti-non-phosphopeptide antibodies
- The animals will be kept after delivery of the antibodies