Phase 1 - Expert consultation
- Definition of the project’s aims and challenges
- Thorough discussion of the antibody characteristics
- Selection of species, isotype, and engineering strategy
Recombinant Antibody Services
Struggling with batch-to-batch variability, limited hybridoma stability, low production rate, or antibody supply issues?
Our recombinant antibody services provide a robust, sequence-defined solution to ensure consistent performance and long-term availability.
At BioGenes, we combine advanced antibody engineering with stable CHO expression systems to deliver high-quality recombinant antibodies tailored to your application.
- Stability: Durable, long-term expression with high reproducibility across batches
- Performance: Increased expression rates up to 30x
- Customization: Highly engineerable (isotype switch, chimerization…)
- Traceability: Defined sequence design
- Full Service Provider: We take care of every step from hybridoma generation to its recombinant expression.
From Hybridoma to Recombinant Antibodies: A Smarter Solution
Recombinant antibodies extend the value of hybridomas by adding greater control, traceability, and long-term reliability. When consistency, documentation, or regulatory readiness become critical, a sequence-defined approach provides a clear advantage.
Our platform enables precise antibody production, including isotype switching and chimerization, combined with transposon-based stable expression in CHO cells. This results in robust cell pools delivering high, consistent antibody yields over time.
Recombinant Antibody Development Strategy
Each project is conducted in close cooperation with the customer and consists of different work phases, each of which can be adapted according to the project’s specific aims.
Phase 2 - Sequence design and vector generation
- Antibody sequence engineering (e.g. chimerization, isotype switch)
- Design and construction of transposon-based expression vectors
- Preparation of high-quality plasmids for efficient cell transfection
Phase 3 - CHO cell line development
- Transfection of CHO cells using transposon systems
- Stable genomic integration and selection of expressing cell pools
Phase 4 - Preservation
- Cryopreservation of selected stable cell pools under controlled conditions
- Long-term storage ensuring genetic and expression stability
Phase 5 - Production
- Antibody expression in CHO system
- Affinity-based purification
- Quality control and characterization of recombinant antibodies
- Antibody labelling (enzyme, biotinylation, fluorophores)
Phase 6 – Delivery
- Delivery of purified antibodies
- Comprehensive documentation (sequence, performance, QC data)
- Technical support and recommendations for downstream applications
Additional Services
Antibody Sequencing Service
Antibody sequencing/hybridoma sequencing allows to determine the variable antibody gene sequences quickly and efficiently conferring the specificity of the antibody. This opens new possibilities in the field of recombinant antibody technologies and permanently establishes antibody identity. Ask us for a antibody sequencing quote at any time.
Test Production
We recommend a test production if there is insufficient information on the production rate of a clone of interest or if you need antibody amounts of 50 mg or more. The test production is based on the affinity purification of the antibody from 350 ml cell culture supernatant.
Large-Scale Antibody Production
BioGenes offers the bulk production of monoclonal antibodies at various scales and quantities ranging from research (mg) to commercial (g) scale amounts per batch. The production is carried out with hybridoma cells specifically developed by BioGenes or with cells provided by the customer.
Longterm Storage and Maintenance
Cell viability and the rate of antibody production from established hybridoma cell lines can diminish over time. In many cases, the antibody production can be restored by re-cloning the hybridoma cell line. The hybridoma re-cloning and development can be ordered separately, if necessary.
- Routine maintenance once every two years (or other time intervals as requested by the customer)
- Includes thawing, re-cultivation and additional cloning
- Screening for high productivity and preparation of new vials for cryo conservation
Further Services & Information
Contact
If you need more information, a specific offer
or want to talk to an expert
FAQs
What are recombinant antibodies?
Recombinant antibodies are engineered immune proteins produced from defined DNA sequences rather than traditional hybridoma methods. By using advanced expression systems such as optimized CHO cell lines, they offer a highly controlled and reproducible alternative to conventional antibodies.
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