Usage of antibodies / storage of antisera and antibodies

Q:

What further processing of antibodies is possible?

A:
  • labelling with different markers (enzymes, fluorescence, gold and biotin)
  • fragmentation and preparation of F(ab) or F(ab)2 fragments
  • preparation of antibody-coated columns for antigen purification
  • coating of ELISA-plates
  • searching for pairs (capturing and detecting antibodies) for developing assays

We can carry out these and other services for you. Please contact us for inquiries or a specific offer.

Q:

Storage of antisera and antibodies

A:

The antisera and/or antibodies are preserved with 0.02% Thimerosal (comparable to Sodium azide) and can be stored at 4°C for a maximum of one month, either in one batch or aliquoted.

The following conditions are recommended for longer storage:

Antisera:

  • aliquoting and freezing at –20 degrees

Antibodies:

  • aliquoting and freezing with 10% glycerol at –20 degrees
  • aliquoting and storing with 30% glycerol at –20 degrees (does not freeze)
  • aliquoting and lyophilising and then storing at 4 degrees

As a general rule, repeated thawing and freezing should be avoided.