Monclonal Antibodies with Guarantee
- Full guarantee without limitation of positive clones
- At least 3 positive stable clones will be guaranteed
Price: 8,200.00 Euro
Duration: 3 to 4 months after ordering and receipt of an antigen.
Money back: If the guarantee cannot be fulfilled on behalf of BioGenes the already paid rate will be paid back in its full extent.
Hybridoma development includes the following steps:
Planning, preparatory work, conjugation, if necessaryImmunisation, cell fusion, selection and screening
- Including immunisation of up to 10 mice, cultivation of the necessary myeloma line, all steps for cell fusion, selection on HAT medium and screening. The customer receives detailed protocols and information concerning the schedule and the tests after conclusion of this step.
- At least two clonings including screening and cultivation of positive cultures after the second cloning. The customer receives 1 ml of supernatant of each positive clone for testing in his own system and confirmation of fulfillment the guarantee.
- This step contains final clonings and all preparatory steps to obtain pure clones. Three cryo cultures will be prepared for each positive stable clone. The cryo cultures and 10 ml of cell culture supernatant of each clone will be delivered to the customer. Furthermore, two cryo cultures can be prepared upon request of the client and be retained for security reasons for at least five years. These cultures will neither be used by BioGenes nor a third party.
- Determination of the Ig subclass and mycoplasma test. A respective certificate and protocol will be provided.
- The customer receives complete documentation of all working steps, and acquires possession of all clones and above mentioned reports with the end of the development.
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