Hybridoma development includes:

(Planning, preparatory work, antigen conjugation, if necessary)

Immunisation, cell fusion, selection and screening
Including immunisation of up to 10 mice, cultivation of the myeloma cell line, all steps for cell fusion, selection in HAT medium and screening. The customer receives detailed  information concerning the work schedule and the results after the screening.

Cloning
Cloning including screening and cultivation of positive cultures will be performed. The customer receives supernatant of each positive clone for testing in his own laboratory and confirmation of the fulfillment of the guarantee.

Final cloning and cryo cultures
Final clonings and all preparatory steps to obtain stable clones. Three cryo cultures will be prepared for each positive stable clone. The cryo cultures and 10 ml of cell culture supernatant of each clone will be delivered to the customer. Furthermore, two more cryo cultures can be prepared upon request that are retained for security reasons for at least five years. These cultures will neither be used by BioGenes nor a third party.

Characterisation of selected clones
Determination of Ig subclass and mycoplasma test. A respective certificate and reportl is provided.

Documentation
Customer receives complete documentation of all working steps and acquires possession of all clones and above mentioned reports with the end of the development.

Please use the questionnaire for planning and specifying the project.